DNA
Part:BBa_K431003:Design
Designed by: Daniel Pingel Group: iGEM10_Georgia_State (2010-06-12)
Pichia pastoris phosphatidylinositol 3-kinase gene, complete cds
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 655
Illegal XbaI site found at 244
Illegal SpeI site found at 1195 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 655
Illegal NheI site found at 484
Illegal SpeI site found at 1195 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 655
Illegal BglII site found at 42
Illegal BglII site found at 68 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 655
Illegal XbaI site found at 244
Illegal SpeI site found at 1195 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 655
Illegal XbaI site found at 244
Illegal SpeI site found at 1195 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Umm 10
Source
1..2904 /organism="Pichia pastoris" /mol_type="genomic DNA" /db_xref="taxon:4922"